smart seq 2 kit (TaKaRa)
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TaKaRa
smart seq 2 kit
Smart Seq 2 Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smart+seq+2+kit/SMARTScribe+Reverse+Transcriptase/pm40490510-441-13-17
Average 96 stars, based on 1128 article reviews
Smart Seq 2 Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smart+seq+2+kit/SMARTScribe+Reverse+Transcriptase/pm40490510-441-13-17
Average 96 stars, based on 1128 article reviews
smart seq 2 kit - by Bioz Stars,
2026-09
96/100 stars
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Lysis:Article Title: Chromothripsis-associated chromosome 21 amplification orchestrates transformation to blast-phase MPN through targetable overexpression of DYRK1A. Article Snippet: In total, 200 CD34+Lin− cells were isolated by FACS and sorted directly into 8 μl of lysis buffer (0.2% Triton X-100, Sigma) containing oligo-dT primers (2.5 μM, IDT, cat. no. 51-01-15-01), dNTP mix (2.5 μM, Life Technologies, cat. no. 19155) and RNase inhibitor (10 U μl−1, Takara (Clontech), cat. no. 2313A) aliquoted into a 96-well PCR plate (Thermo Fisher, segmented semi-skirted, cat. no. AB-0900) on ice. .. Cell lysis, reverse transcription and PCR amplification (20 cycles) were performed using the Article Title: Chromothripsis-associated chromosome 21 amplification orchestrates transformation to blast-phase MPN through targetable overexpression of DYRK1A Article Snippet: In total, 200 CD34 + Lin − cells were isolated by FACS and sorted directly into 8 μl of lysis buffer (0.2% Triton X-100, Sigma) containing oligo-dT primers (2.5 μM, IDT, cat. no. 51-01-15-01), dNTP mix (2.5 μM, Life Technologies, cat. no. 19155) and RNase inhibitor (10 U μl −1 , Takara (Clontech), cat. no. 2313A) aliquoted into a 96-well PCR plate (Thermo Fisher, segmented semi-skirted, cat. no. AB-0900) on ice. .. Cell lysis, reverse transcription and PCR amplification (20 cycles) were performed using the Reverse Transcription:Article Title: Chromothripsis-associated chromosome 21 amplification orchestrates transformation to blast-phase MPN through targetable overexpression of DYRK1A. Article Snippet: In total, 200 CD34+Lin− cells were isolated by FACS and sorted directly into 8 μl of lysis buffer (0.2% Triton X-100, Sigma) containing oligo-dT primers (2.5 μM, IDT, cat. no. 51-01-15-01), dNTP mix (2.5 μM, Life Technologies, cat. no. 19155) and RNase inhibitor (10 U μl−1, Takara (Clontech), cat. no. 2313A) aliquoted into a 96-well PCR plate (Thermo Fisher, segmented semi-skirted, cat. no. AB-0900) on ice. .. Cell lysis, reverse transcription and PCR amplification (20 cycles) were performed using the Article Title: Chromothripsis-associated chromosome 21 amplification orchestrates transformation to blast-phase MPN through targetable overexpression of DYRK1A Article Snippet: In total, 200 CD34 + Lin − cells were isolated by FACS and sorted directly into 8 μl of lysis buffer (0.2% Triton X-100, Sigma) containing oligo-dT primers (2.5 μM, IDT, cat. no. 51-01-15-01), dNTP mix (2.5 μM, Life Technologies, cat. no. 19155) and RNase inhibitor (10 U μl −1 , Takara (Clontech), cat. no. 2313A) aliquoted into a 96-well PCR plate (Thermo Fisher, segmented semi-skirted, cat. no. AB-0900) on ice. .. Cell lysis, reverse transcription and PCR amplification (20 cycles) were performed using the Polymerase Chain Reaction:Article Title: Chromothripsis-associated chromosome 21 amplification orchestrates transformation to blast-phase MPN through targetable overexpression of DYRK1A. Article Snippet: In total, 200 CD34+Lin− cells were isolated by FACS and sorted directly into 8 μl of lysis buffer (0.2% Triton X-100, Sigma) containing oligo-dT primers (2.5 μM, IDT, cat. no. 51-01-15-01), dNTP mix (2.5 μM, Life Technologies, cat. no. 19155) and RNase inhibitor (10 U μl−1, Takara (Clontech), cat. no. 2313A) aliquoted into a 96-well PCR plate (Thermo Fisher, segmented semi-skirted, cat. no. AB-0900) on ice. .. Cell lysis, reverse transcription and PCR amplification (20 cycles) were performed using the Article Title: Chromothripsis-associated chromosome 21 amplification orchestrates transformation to blast-phase MPN through targetable overexpression of DYRK1A Article Snippet: In total, 200 CD34 + Lin − cells were isolated by FACS and sorted directly into 8 μl of lysis buffer (0.2% Triton X-100, Sigma) containing oligo-dT primers (2.5 μM, IDT, cat. no. 51-01-15-01), dNTP mix (2.5 μM, Life Technologies, cat. no. 19155) and RNase inhibitor (10 U μl −1 , Takara (Clontech), cat. no. 2313A) aliquoted into a 96-well PCR plate (Thermo Fisher, segmented semi-skirted, cat. no. AB-0900) on ice. .. Cell lysis, reverse transcription and PCR amplification (20 cycles) were performed using the Amplification:Article Title: Chromothripsis-associated chromosome 21 amplification orchestrates transformation to blast-phase MPN through targetable overexpression of DYRK1A. Article Snippet: In total, 200 CD34+Lin− cells were isolated by FACS and sorted directly into 8 μl of lysis buffer (0.2% Triton X-100, Sigma) containing oligo-dT primers (2.5 μM, IDT, cat. no. 51-01-15-01), dNTP mix (2.5 μM, Life Technologies, cat. no. 19155) and RNase inhibitor (10 U μl−1, Takara (Clontech), cat. no. 2313A) aliquoted into a 96-well PCR plate (Thermo Fisher, segmented semi-skirted, cat. no. AB-0900) on ice. .. Cell lysis, reverse transcription and PCR amplification (20 cycles) were performed using the Article Title: Chromothripsis-associated chromosome 21 amplification orchestrates transformation to blast-phase MPN through targetable overexpression of DYRK1A Article Snippet: In total, 200 CD34 + Lin − cells were isolated by FACS and sorted directly into 8 μl of lysis buffer (0.2% Triton X-100, Sigma) containing oligo-dT primers (2.5 μM, IDT, cat. no. 51-01-15-01), dNTP mix (2.5 μM, Life Technologies, cat. no. 19155) and RNase inhibitor (10 U μl −1 , Takara (Clontech), cat. no. 2313A) aliquoted into a 96-well PCR plate (Thermo Fisher, segmented semi-skirted, cat. no. AB-0900) on ice. .. Cell lysis, reverse transcription and PCR amplification (20 cycles) were performed using the |